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Publications

2018

  • Structure-Function Relationship of XCL1 Used for in vivo Targeting of Antigen Into XCR1+ Dendritic Cells.
    Kroczek AL, Hartung E, Gurka S, Becker M, Reeg N, Mages HW, Voigt S, Freund C, Kroczek RA.
    Front Immunol. 2018 Dec 13;9:2806. doi: 10.3389/fimmu.2018.02806. eCollection 2018.

  • Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells.
    Waldt N, Seifert A, Demiray YE, Devroe E, Turk BE, Reichardt P, Mix C, Reinhold A, Freund C, Müller AJ, Schraven B, Stork O, Kliche S.
    Front Immunol. 2018 Dec 4;9:2852. doi: 10.3389/fimmu.2018.02852. eCollection 2018.

  • Distinct editing functions of natural HLA-DM allotypes impact antigen presentation and CD4+ T cell activation.
    Álvaro-Benito M, Morrison E, Ebner F, Abualrous ET, Urbicht M, Wieczorek M, Freund C.
    Cell Mol Immunol. 2018 Nov 22. doi: 10.1038/s41423-018-0181-1. [Epub ahead of print]

  • The GTPase ARFRP1 affects lipid droplet protein composition and triglyceride release from intracellular storage of intestinal Caco-2 cells.
    Werno MW, Wilhelmi I, Kuropka B, Ebert F, Freund C, Schürmann A.
    Biochem Biophys Res Commun. 2018 Oct 19. pii: S0006-291X(18)32248-4. doi: 10.1016/j.bbrc.2018.10.092. [Epub ahead of print]

  • Understanding the elusive protein corona of thermoresponsive nanogels.
    Miceli E, Kuropka B, Rosenauer C, Osorio Blanco ER, Theune LE, Kar M, Weise C, Morsbach S, Freund C, Calderón M.
    Nanomedicine (Lond). 2018 Oct 18. doi: 10.2217/nnm-2018-0217. [Epub ahead of print]

  • Quantification of HLA-DM-Dependent Major Histocompatibility Complex of Class II Immunopeptidomes by the Peptide Landscape Antigenic Epitope Alignment Utility.
    Álvaro-Benito M, Morrison E, Abualrous ET, Kuropka B, Freund C.
    Front Immunol. 2018 May 3;9:872. doi: 10.3389/fimmu.2018.00872. eCollection 2018.

  • Intrinsically Disordered Protein Ntr2 Modulates the Spliceosomal RNA Helicase Brr2.
    Wollenhaupt J, Henning LM, Sticht J, Becke C, Freund C, Santos KF, Wahl MC.
    Biophys J. 2018 Feb 27;114(4):788-799. doi: 10.1016/j.bpj.2017.12.033.

2017

  • A Coincidence Detection Mechanism Controls PX-BAR Domain-Mediated Endocytic Membrane Remodeling via an Allosteric Structural Switch.
    Lo WT, Vujičić Žagar A, Gerth F, Lehmann M, Puchkov D, Krylova O, Freund C, Scapozza L, Vadas O, Haucke V.
    Dev Cell. 2017; 43, 522-529.

  • Intersectin associates with synapsin and regulates its nanoscale localization and function.
    Gerth F, Jäpel M, Pechstein A, Kochlamazashvili G, Lehmann M, Puchkov D, Onofri F, Benfenati F, Nikonenko AG, Fredrich K, Shupliakov O, Maritzen T, Freund C, Haucke V.
    Proc Natl Acad Sci U S A. 2017; 114, 12057-12062.

  • Protein-peptide association kinetics beyond the seconds timescale from atomistic simulations.
    Paul F, Wehmeyer C, Abualrous ET, Wu H, Crabtree MD, Schöneberg J, Clarke J, Freund C, Weikl TR, Noé F.
    Nat. Commun. 2017 8, 1095.

  • A new role for FBP21 as regulator of Brr2 helicase activity.
    Henning LM, Santos KF, Sticht J, Jehle S, Lee CT, Wittwer M, Urlaub H, Stelzl U, Wahl MC, Freund C.
    Nucleic Acids Res. 2017; 45, 7922-7937.

  • Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.
    Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W.
    Elife 2017; 6. pii: e28985.

  • The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells.
    Niemz J, Kliche S, Pils MC, Morrison E, Manns A, Freund C, Crittenden JR, Graybiel AM, Galla M, Jänsch L, Huehn J.
    Eur J Microbiol Immunol 2017; (Bp). 7, 112-126.

  • Identification of sortase substrates by specificity profiling.
    Schmohl L, Bierlmeier J, von Kügelgen N, Kurz L, Reis P, Barthels F, Mach P, Schutkowski M, Freund C, Schwarzer D.
    Bioorg Med Chem. 2017; 25, 5002-5007.

  • Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
    Wieczorek M, Abualrous ET, Sticht J, Álvaro-Benito M, Stolzenberg S, Noé F, Freund C.
    Front Immunol. 2017; 8, 292.

  • Noninvasive Imaging of Human Immune Responses in a Human Xenograft Model of Graft-Versus-Host Disease.
    Van Elssen CHMJ, Rashidian M, Vrbanac V, Wucherpfennig KW, Habre ZE, Sticht J, Freund C, Jacobsen JT, Cragnolini J, Ingram J, Plaisier L, Spierings E, Tager AM, Ploegh HL.
    J Nucl. Med 2017; 58(6):1003-1008.

  • Engineering sortase A by screening a second-generation library using phage display.
    Schmohl L, Bierlmeier J, Gerth F, Freund C, Schwarzer D.
    J Pept Sci. 2017; 10.1002/psc.2980

  • The differentiation and plasticity of Tc17 cells are regulated by CTLA-4-mediated effects on STATs.
    Arra A, Lingel H, Kuropka B, Pick J, Schnoeder T, Fischer T, Freund C, Pierau M, Brunner-Weinzierl MC.
    Oncoimmunology 2017; 6(2): e1273300.

  • D120 and K152 within the PH Domain of T Cell Adapter SKAP55 Regulate Plasma Membrane Targeting of SKAP55 and LFA-1 Affinity Modulation in Human T Lymphocytes.
    Witte A, Meineke B, Sticht J, Philipsen L, Kuropka B, Müller AJ, Freund C, Schraven B, Kliche S.
    Mol Cell Biol 2017; 37(7): e00509-16.

2016

  • MHC class II complexes sample intermediate states along the peptide exchange pathway.
    Wieczorek M, Sticht J, Stolzenberg S, Günther S, Wehmeyer C, El Habre Z, Álvaro-Benito M, Noé F, Freund C.
    Nat Commun. 2016 Nov 9;7:13224.

  • Human leukocyte Antigen-DM polymorphisms in autoimmune diseases.
    Alvaro-Benito M, Morrison E, Wieczorek M, Sticht J, Freund C.
    Open Biol.     2016 Aug;6(8).

  • Tyrosine-phosphorylation of the scaffold protein ADAP and its role in T cell signaling.
    Kuropka B, Schraven B, Kliche S, Krause E, Freund C
    Expert Rev Proteomics    . 2016 Jun;13(6):545-54.

2015

  • Analysis of Phosphorylation-dependent Protein Interactions of Adhesion and Degranulation Promoting Adaptor Protein (ADAP) Reveals Novel Interaction Partners Required for Chemokine-directed T cell Migration.
    Kuropka B, Witte A, Sticht J, Waldt N, Majkut P, Hackenberger CP, Schraven B, Krause E, Kliche S, Freund C
    Mol Cell Proteomics.     2015 Nov 1; 14:2961-72.

  • The GYF domain protein CD2BP2 is critical for embryogenesis and podocyte function.
    Albert GI, Schell C, Kirschner KM, Schäfer S, Naumann R, Müller A, Kretz O, Kuropka B, Girbig M, Hübner N, Krause E, Scholz H, Huber TB, Knobeloch KP, Freund C
    J Mol Cell Biol.     2015 Oct; 7:402-14

  • Quantitative analysis of the human T cell palmitome.
    Morrison, E., Kuropka, B., Kliche, S., Krause, E. and Freund, C.
    Sci. Rep.    , 2015 Jun 26;5:11598.

  • Peptide-polymer ligands for a tandem WW-domain, an adaptive multivalent protein-protein interaction: lessons on the thermodynamic fitness of flexible ligands.
    Koschek K, Durmaz V, Krylova O, Wieczorek M, Gupta S, Richter M, Bujotzek A, Fischer C, Haag R, Freund C, Weber M, Rademann J.
    Beilstein J Org Chem.    , 2015 May 18;11:837-47.

  • Exploring monovalent and multivalent peptides for the inhibition of FBP21-tWW.
    Henning, L. M., Bhatia, S., Bertazzon, M., Marczynke, M., Seitz, O., Volkmer, R., Haag, R. and Freund, C.
    Beilstein J. Org. Chem.    ,  2015 May 11;11:701-6.

  • ProMs: A modular toolkit to inhibit proline-rich motif mediated protein-protein interactions.
    Opitz, R., Müller, M., , C., Barone, M., Soicke, A., Roske, Y., Piotukh, K., Huy, P., Beerbaum, M., Wiesner, B., Beyermann, M., Schmieder, P., Freund, C., Volkmer, R., Oschkinat, H., Schmalz, H.-G. & Kühne, R.
    Proc. Natl. Acad. Sci.     (USA), 2015 Apr 21;112(16):5011-6.

  • Sortase A mediated site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments.
    Kuropka B, Royla N, Freund C, Krause E.
    Proteomics    . 2015 Apr;15(7):1230-4.

  • Vesicle uncoating regulated by SH3-SH3 domain-mediated complex formation between endophilin and intersectin at synapses
    Pechstein, A., Gerth, F., Milosevic, I., Jäpel, M., Eichhorn-Grünig, M., Vorontsova, O., Bacetic, J., Maritzen, T., Shupliakov, O., Freund, C. & Haucke, V.
    EMBO Rep. 2015 Feb;16(2):232-9.

  • HLA-DMA polymorphisms differentially affect MHC class II peptide loading.
    Álvaro-Benito, M., Wieczorek, M., Sticht, J., Kipar, C. & Freund, C.
    J Immunol. 2015 Jan 15;194(2):803-16.

2014

  • Multichannel MRI Labeling of Mammalian Cells by Switchable Nanocarriers for Hyperpolarized Xenon.
    Klippel S, Freund C, Schröder L.
    Nano Lett. 2014 Sep 30. [Epub ahead of print]

  • Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
    Rose HM, Witte C, Rossella F, Klippel S, Freund C, Schröder L.
    Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11697-702.

  • Susceptibility to HLA-DM Protein Is Determined by a Dynamic Conformation of Major Histocompatibility Complex Class II Molecule Bound with Peptide.
    Yin L, Trenh O, Wieczorek M, Lange S, Sticht J, Jiang W, Bylsma M, Mellins ED, Freund C, Stern LJ.
    J Biol Chem.     2014 Aug 22; 289(34):23449-64

  • The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth.
    Rehberg K, Kliche S, Madencioglu DA, Thiere M, Müller B, Meineke BM, Freund C, Budinger E, Stork O.
    J Neurosci. 2014 Apr 9;34(15):5342-54.

2013

  • Completion of proteomic data sets by Kd measurement using cell-free synthesis of site-specifically labeled proteins.
    Majkut P, Claußnitzer I, Merk H, Freund C, Hackenberger CP, Gerrits M.
    PLoS One. 2013 Dec 10;8(12):e82352.

  • Cell tracking with caged xenon: using cryptophanes as MRI reporters upon cellular internalization.
    Klippel S, Döpfert J, Jayapaul J, Kunth M, Rossella F, Schnurr M, Witte C, Freund C, Schröder L.
    Angew Chem Int Ed Engl. 2014 Jan 7;53(2):493-6.

  • Probing the recognition of post-translational modifications by combining sortase-mediated ligation and phage-assisted selection.
    Teschke T, Geltinger B, Dose A, Freund C, Schwarzer D.
    ACS Chem Biol. 2013 Aug 16;8(8):1692-7.

2012

  • Phosphatidylinositol 4-kinase II function at endosomes is regulated by the ubiquitin ligase Itch.
    Mössinger, J., Wieffer, M., Krause, E., Freund, C., Gerth, F., Krauss, M. & Haucke, V.
    EMBO Reports 13, 1087-1094.

  • A novel hSH3 domain scaffold engineered to bind folded domains in CD2BP2 and HIV capsid protein.
    Piotukh, K. & Freund, C.
    Protein Eng. Des. Sel. 2012, 25:649-56.

  • Peptide linkage to the alpha-subunit of MHCII creates a stably inverted antigen presentation complex.
    Schlundt, A., Günther, S., Sticht, J., Wieczorek, M., Roske, Y., Heinemann, U. & Freund, C.
    J. Mol. Biol. 2012, 423, 294-302.

  • CCR7-mediated LFA-1 functions in T cells are regulated by 2 independent ADAP/SKAP55 modules.
    Kliche S, Worbs T, Wang X, Degen J, Patzak I, Meineke B, Togni M, Moser M, Reinhold A, Kiefer F, Freund C, Förster R, Schraven B.
    Blood. 2012 Jan 19;119(3):777-85. Epub 2011 Nov 23. PMID:22117043

  • An improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions.
    Stephanowitz, H., Lange, S., Lang, D., Freund, C., and Krause, E.
    J. Proteome Research 2012, 11(2):1175-83.

2011

  • Directed evolution of sortase A mutants with altered substrate selectivity profiles.
    Piotukh K, Geltinger B, Heinrich N, Gerth F, Beyermann M, Freund C, Schwarzer D.
    J Am Chem Soc. 2011 Nov 9;133(44):17536-9. Epub 2011 Oct 13.     

  • Multivalent binding of formin-binding protein 21 (FBP21)-tandem-WW domains fosters protein recognition in the pre-spliceosome.
    Klippel S, Wieczorek M, Schümann M, Krause E, Marg B, Seidel T, Meyer T, Knapp EW, Freund C.
    J Biol Chem. 2011 Nov 4;286(44):38478-87. Epub 2011 Sep 14.      

  • Characterization of structural features controlling the receptiveness of empty class II MHC molecules.
    Rupp B, Günther S, Makhmoor T, Schlundt A, Dickhaut K, Gupta S, Choudhary I, Wiesmüller KH, Jung G, Freund C, Falk K, Rötzschke O, Kühne R.
    PLoS One. 2011 Apr 14;6(4):e18662.     

  • Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.
    Borja MS, Piotukh K, Freund C, Gross JD.
    RNA. 2011 Feb;17(2):278-90. Epub 2010 Dec 10.

2010

  • Bidirectional binding of invariant chain peptides to an MHC class II molecule.
    Günther S, Schlundt A, Sticht J, Roske Y, Heinemann U, Wiesmüller KH, Jung G, Falk K, Rötzschke O, Freund C.
    Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22219-24. Epub 2010 Nov 29.     

  • Addressing protein-protein interactions with small molecules: a Pro-Pro dipeptide mimic with a PPII helix conformation as a module for the synthesis of PRD-binding ligands.
    Zaminer J, Brockmann C, Huy P, Opitz R, Reuter C, Beyermann M, Freund C, Müller M, Oschkinat H, Kühne R, Schmalz HG.
    Angew Chem Int Ed Engl. 2010 Sep 17;49(39):7111-5.     

  • Conserved beta-hairpin recognition by the GYF domains of Smy2 and GIGYF2 in mRNA surveillance and vesicular transport complexes.
    Ash MR, Faelber K, Kosslick D, Albert GI, Roske Y, Kofler M, Schuemann M, Krause E, Freund C.
    Structure. 2010 Aug 11;18(8):944-54.     

  • Adhesion and degranulation promoting adapter protein (ADAP) is a central hub for phosphotyrosine-mediated interactions in T cells.
    Sylvester M, Kliche S, Lange S, Geithner S, Klemm C, Schlosser A, Grossmann A, Stelzl U, Schraven B, Krause E, Freund C.
    PLoS One. 2010 Jul 22;5(7):e11708. Erratum in: PLoS One. 2010;5(8).

  • Identification of phosphorylation-dependent interaction partners of the adapter protein ADAP using quantitative mass spectrometry: SILAC vs (18)O-labeling.
    Lange S, Sylvester M, Schümann M, Freund C, Krause E.
    J Proteome Res. 2010 Aug 6;9(8):4113-22.

2009

  • A novel S3S-TAP-tag for the isolation of T-cell interaction partners of adhesion and degranulation promoting adaptor protein.
    Lehmann R, Meyer J, Schuemann M, Krause E, Freund C.
    Proteomics. 2009 Dec;9(23):5288-95.

  • Proline-rich sequence recognition: II. Proteomics analysis of Tsg101 ubiquitin-E2-like variant (UEV) interactions.
    Schlundt A, Sticht J, Piotukh K, Kosslick D, Jahnke N, Keller S, Schuemann M, Krause E, Freund C.
    Mol Cell Proteomics. 2009 Nov;8(11):2474-86.

  • Proline-rich sequence recognition: I. Marking GYF and WW domain assembly sites in early spliceosomal complexes.
    Kofler M, Schuemann M, Merz C, Kosslick D, Schlundt A, Tannert A, Schaefer M, Lührmann R, Krause E, Freund C.
    Mol Cell Proteomics. 2009 Nov;8(11):2461-73.

  • A xenon-129 biosensor for monitoring MHC-peptide interactions.
    Schlundt A, Kilian W, Beyermann M, Sticht J, Günther S, Höpner S, Falk K, Roetzschke O, Mitschang L, Freund C.
    Angew Chem Int Ed Engl. 2009;48(23):4142-5.

2008

  • Resin-bound aminofluorescein for C-terminal labeling of peptides: high-affinity polarization probes binding to polyproline-specific GYF domains.
    Uryga-Polowy V, Kosslick D, Freund C, Rademann J.
    Chembiochem. 2008 Oct 13;9(15):2452-62.

  • Proline-rich sequence recognition domains (PRD): ligands, function and inhibition.
    Freund C, Schmalz HG, Sticht J, Kühne R.
    Handb Exp Pharmacol. 2008;(186):407-29. Review.

  • Mutational analyses of c-FLIPR, the only murine short FLIP isoform, reveal requirements for DISC recruitment.
    Ueffing N, Keil E, Freund C, Kühne R, Schulze-Osthoff K, Schmitz I.
    Cell Death Differ. 2008 Apr;15(4):773-82. 

  • Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays.
    Rathert P, Zhang X, Freund C, Cheng X, Jeltsch A.
    Chem Biol. 2008 Jan;15(1):5-11.

2007

  • Investigating the functional role of CD2BP2 in T cells.
    Heinze M, Kofler M, Freund C.
    Int Immunol. 2007 Nov;19(11):1313-8.

  • Reversible disulfide bond formation of intracellular proteins probed by NMR spectroscopy.
    Piotukh K, Kosslick D, Zimmermann J, Krause E, Freund C.
    Free Radic Biol Med. 2007 Nov 1;43(9):1263-70.

  • Redox-regulated conformational changes in an SH3 domain.
    Zimmermann J, Kühne R, Sylvester M, Freund C.
    Biochemistry. 2007 Jun 12;46(23):6971-7.

2006

  • Small organic compounds enhance antigen loading of class II major histocompatibility complex proteins by targeting the polymorphic P1 pocket.
    Höpner S, Dickhaut K, Hofstätter M, Krämer H, Rückerl D, Söderhäll JA, Gupta S, Marin-Esteban V, Kühne R, Freund C, Jung G, Falk K, Rötzschke O.
    J Biol Chem. 2006 Dec 15;281(50):38535-42.  

  • The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1.
    Kliche S, Breitling D, Togni M, Pusch R, Heuer K, Wang X, Freund C, Kasirer-Friede A, Menasche G, Koretzky GA, Schraven B.
    Mol Cell Biol. 2006 Oct;26(19):7130-44.

  • Lipid-binding hSH3 domains in immune cell adapter proteins.
    Heuer K, Sylvester M, Kliche S, Pusch R, Thiemke K, Schraven B, Freund C.
    J Mol Biol. 2006 Aug 4;361(1):94-104.

  • The GYF domain.
    Kofler MM, Freund C.
    FEBS J. 2006 Jan;273(2):245-56. Review.

2005

  • GYF domain proteomics reveals interaction sites in known and novel target proteins.
    Kofler M, Motzny K, Freund C.
    Mol Cell Proteomics. 2005 Nov;4(11):1797-811.

  • NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain.
    Zimmermann J, Freund C.
    J Biomol NMR. 2005 May;32(1):94.

  • Novel interaction partners of the CD2BP2-GYF domain.
    Kofler M, Motzny K, Beyermann M, Freund C.
    J Biol Chem. 2005 Sep 30;280(39):33397-402.

  • Alternative binding modes of proline-rich peptides binding to the GYF domain.
    Gu W, Kofler M, Antes I, Freund C, Helms V.
    Biochemistry. 2005 May 3;44(17):6404-15.

  • Cyclophilin A binds to linear peptide motifs containing a consensus that is present in many human proteins.
    Piotukh K, Gu W, Kofler M, Labudde D, Helms V, Freund C.
    J Biol Chem. 2005 Jun 24;280(25):23668-74.

  • The helically extended SH3 domain of the T cell adaptor protein ADAP is a novel lipid interaction domain.
    Heuer K, Arbuzova A, Strauss H, Kofler M, Freund C.
    J Mol Biol. 2005 May 13;348(4):1025-35.

  • Statistically significant dependence of the Xaa-Pro peptide bond conformation on secondary structure and amino acid sequence.
    Pahlke D, Freund C, Leitner D, Labudde D.
    BMC Struct Biol. 2005 Apr 1;5:8.

2004

  • Recognition sequences for the GYF domain reveal a possible spliceosomal function of CD2BP2.
    Kofler M, Heuer K, Zech T, Freund C.
    J Biol Chem. 2004 Jul 2;279(27):28292-7.

  • Structure of a helically extended SH3 domain of the T cell adapter protein ADAP.
    Heuer K, Kofler M, Langdon G, Thiemke K, Freund C.
    Structure. 2004 Apr;12(4):603-10.