Human sulfotransferases (SULTs) are phase ІІ enzymes which catalyze the transferring of sulfo-moieties to alcoholic or amino acceptors. In my study, we successfully expressed 14 human SULTs using recombinant fission yeast cells. A modified variant of sulfotransferase assay was developed that employs permeabilized fission yeast cells (enzyme bags). The enzymatic functionality of SULT4A1 and SULT6B1 was first demonstrated in our research. Currently, an optimized approach for reaching a higher metabolite yield with lower cost are undergoing developing. Further multi-substrates screening or up-scaling will be performed based on the optimized method.